In vitro protocols for germplasm preservation

The OPGC is attempting to back-up in vitro its entire clonal collection (primarily of Pelargonium and Begonia).  Various approaches are being explored to introduce the accessions in culture and then establish methods for slow growth to reduce the frequency of subculture needed to keep the material alive.  In addition, the Phlox collection has also been introduced in vitro while various issues associated with seed production and seed qualit are examined.  The research involves alterations of nutrient media, addition of osmoticum, modification of light quality, and reduction in temperature.