The grounds surrounding the OPGC building and greenhouse are used to grow plants in containers or in raised beds. We also have a poly-house to control moisture in plants that prefer drier conditions.
The grounds surrounding the main building and greenhouse of the OPGC are used to grow plants in raised beds or in containers.
Our small wet lab allows us to prepare material for in vitro culture as well as isolate nuclei for DNA content by flow cytometry and also for simple molecular analyses.
We use the 20C culture room to establish plants in vitro and to manage initial clonal collections before eventual storage at 4C.
We are exploring the use of 4C conditions to maintain germplasm in order to slow down growth and reduce the frequency of subcultures.
The laminar flow hoods at the OPGC are routine used for subculture of in-vitro accessions or for various studies such as the one illustrated here where mature embryos of Phlox seed are being extracted to assess germination capacity.
The autoclave is used regularly to sterilize our media and materials.
The 20C culture room is used to establish cultures and maintain those accessions that do not tolerate 4C.
The largest indoor space in our building is used for seed conditioning where we can use various methods to clean seeds prior to viability assessment and eventual storage.
We are able to clean some of our seeds using vibratory tables that separate seeds by geometry and size.
We have a thermogradient table that allows us to evaluate germination of seeds at different temperatures in the presence or absence of light.